- Adamov
Healthy Cities Network member since 2007
- Benešov
Healthy Cities Network member since 2016
- Benešov nad Ploučnicí
Healthy Cities Network member since 2015
- Bolatice
Healthy Cities Network member since 2006
- Bory
Healthy Cities Network member since 2012
- Boskovice
Healthy Cities Network member since 1994
- Brandýs nad Labem - Stará Boleslav
Healthy Cities Network member since 2012
- Břeclav
Healthy Cities Network member since 2011
- Březina
Healthy Cities Network member since 2015
- Brno
Healthy Cities Network member since 1994
- Bystřice
Healthy Cities Network member since 2017
- Chelčice
Healthy Cities Network member since 2014
- Chrudim
Healthy Cities Network member since 2001
- Červená Voda
Healthy Cities Network member since 2017
- Český Brod
Healthy Cities Network member since 2009
- Dačice
Healthy Cities Network member since 2011
- Dobříš
Healthy Cities Network member since 2008
- Havlíčkův Brod
Healthy Cities Network member since 2016
- Hlučín
Healthy Cities Network member since 2007
- Hodonín
Healthy Cities Network member since 1998
- Humpolec
Healthy Cities Network member since 2005
- Jesenice
Healthy Cities Network member since 2012
- Jihlava
Healthy Cities Network member since 2007
- Jilemnice
Healthy Cities Network member since 2002
- Kácov
Healthy Cities Network member since 2007
- Karlovice
Healthy Cities Network member since 2011
- Klatovy
Healthy Cities Network member since 2011
- Koleč
Healthy Cities Network member since 2008
- Kopřivnice
Healthy Cities Network member since 2003
- Kouřim
Healthy Cities Network member since 1998
- Krásno
Healthy Cities Network member since 2015
- Krhová
Healthy Cities Network member since 2016
- Křídla
Healthy Cities Network member since 2012
- Křižánky
Healthy Cities Network member since 2006
- Křižany
Healthy Cities Network member since 2005
- Krnov
Healthy Cities Network member since 2012
- Kroměříž
Healthy Cities Network member since 1996
- Letovice
Healthy Cities Network member since 2000
- Liberec
Healthy Cities Network member since 1996
- Lípa
Healthy Cities Network member since 2016
- Litoměřice
Healthy Cities Network member since 2000
- Lomnice nad Popelkou
Healthy Cities Network member since 2007
- Mariánské Lázně
Healthy Cities Network member since 2015
- Mladá Boleslav
Healthy Cities Network member since 2003
- Moravská Třebová
Healthy Cities Network member since 1997
- Mšeno
Healthy Cities Network member since 2010
- Nové Město na Moravě
Healthy Cities Network member since 2001
- Nový Jičín
Healthy Cities Network member since 2015
- Opava
Healthy Cities Network member since 2011
- Orlová
Healthy Cities Network member since 1997
- Pelhřimov
Healthy Cities Network member since 2005
- Pištín
Healthy Cities Network member since 2014
- Počátky
Healthy Cities Network member since 2010
- Poděbrady
Healthy Cities Network member since 1997
- Prachatice
Healthy Cities Network member since 2009
- Praha 04
Healthy Cities Network member since 2014
- Praha 07
Healthy Cities Network member since 2008
- Praha 08
Healthy Cities Network member since 2016
- Praha 10
Healthy Cities Network member since 2016
- Praha 12
Healthy Cities Network member since 2015
- Praha 15
Healthy Cities Network member since 2017
- Praha 18
Healthy Cities Network member since 2009
- Praha 19
Healthy Cities Network member since 2010
- Praha 20
Healthy Cities Network member since 2013
- Praha 21
Healthy Cities Network member since 2012
- Praha-Dolní Počernice
Healthy Cities Network member since 2008
- Praha-Libuš
Healthy Cities Network member since 2005
- Praha-Slivenec
Healthy Cities Network member since 2008
- Přeštice
Healthy Cities Network member since 2007
- Prostějov
Healthy Cities Network member since 2000
- Rokytnice nad Jizerou
Healthy Cities Network member since 2015
- Rosice
Healthy Cities Network member since 2015
- Rožmitál pod Třemšínem
Healthy Cities Network member since 2001
- Rožnov pod Radhoštěm
Healthy Cities Network member since 2004
- Rudíkov
Healthy Cities Network member since 2014
- Sázava
Healthy Cities Network member since 2015
- Skotnice
Healthy Cities Network member since 2003
- Strančice
Healthy Cities Network member since 2010
- Strašín
Healthy Cities Network member since 2005
- Strážek
Healthy Cities Network member since 2006
- Šlapanice
Healthy Cities Network member since 2013
- Šternberk
Healthy Cities Network member since 1994
- Štětí
Healthy Cities Network member since 2003
- Tábor
Healthy Cities Network member since 2008
- Telč
Healthy Cities Network member since 2004
- Telnice
Healthy Cities Network member since 2015
- Třebíč
Healthy Cities Network member since 1996
- Třeboň
Healthy Cities Network member since 1994
- Turnov
Healthy Cities Network member since 2011
- Týnec nad Sázavou
Healthy Cities Network member since 2016
- Uherský Brod
Healthy Cities Network member since 2005
- Ústí nad Labem
Healthy Cities Network member since 2005
- Úštěk
Healthy Cities Network member since 2015
- Valašské Klobouky
Healthy Cities Network member since 2015
- Valašské Meziříčí
Healthy Cities Network member since 2000
- Velké Meziříčí
Healthy Cities Network member since 2001
- Velký Beranov
Healthy Cities Network member since 2007
- Věžnice
Healthy Cities Network member since 2013
- Vítkov
Healthy Cities Network member since 2015
- Vřesina
Healthy Cities Network member since 2015
- Vsetín
Healthy Cities Network member since 2001
- Vyžlovka
Healthy Cities Network member since 2016
- Zákolany
Healthy Cities Network member since 2011
- Zbyslavice
Healthy Cities Network member since 2015
- Zlaté Hory
Healthy Cities Network member since 1999
- Znojmo
Healthy Cities Network member since 2013
